The expression of GUCA2a, GUCA2b and GUCY2 genes was higher in jejunum, ileum, descending colon and rectum.
A common pattern of expression throughout the gastrointestinal lumen for all three investigated transcripts was found. Additionally, the protein expression levels of GC-C receptor were analyzed through western blotting. Data analysis was carried out with Bio-Rad CFX Manager software, and genes of interest normalized relative to the abundance of the two reference genes ( SDHA, HPRT). For each sample, total RNA was extracted from 100 mg of ground tissue, and qRT-PCR performed on GUCA2a, GUCA2b and GUCY2 transcripts on a CFX96 Touch instrument. Tissue samples from stomach, duodenum, jejunum, ileum, head and body of cecum, left and right dorsal colon, left and right ventral colon, pelvic flexure, transverse colon, descending colon and rectum were collected from adult horses within 1 h post mortem. We investigated for the first time the gene expression of guanylin, uroguanylin and GC-C receptors in different horse’s gastrointestinal tracts. Little is known about this system in horses. Guanylate cyclase-C (GC-C) is a multifunctional receptor encoded by the GUCY2C gene, representing an attractive target for therapy in several gastrointestinal diseases in humans. 4Department of Experimental Medicine, School of Medicine, University of Perugia, Perugia, Italy.3Department of Medicine, School of Medicine, University of Perugia, Perugia, Italy.
2School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy.1Department of Veterinary Medicine, University of Perugia, Perugia, Italy.Katia Cappelli 1†, Rodolfo Gialletti 1†, Beniamino Tesei 2, Gabrio Bassotti 3, Katia Fettucciari 4, Stefano Capomaccio 1, Laura Bonfili 2, Massimiliano Cuccioloni 2, Anna Maria Eleuteri 2, Andrea Spaterna 2 and Fulvio Laus 2*
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